The kits are developed according to the norms ISO22119, ISO20838, ISO16140 hence they include an internal amplification control, a positive and a negative control. This kit contains two 3-plex mixes detecting MIX1 [Salmonella spp. (FAM) IAC (HEX) L.monocytogenes (TxRed)] - MIX 2 [stx1 + stx2 (FAM) IPC (HEX) eae (TxRed)]. When paired to an appropriate DNA extraction kit (e.g. Generon FASTfood - Cat# EXD009) the kit allows, at the same time, for the detection of Salmonella, Listeria Monocytogenes and STECs in less than 2 hours. Salmonella, STECs and Listeria Monocytogenes can be detected in pools of two selective enriched media broth (e.g. BPW and Fraser) or in water samples collection filters. The kit in less than 30 minutes, without any DNA extraction step, allows also for the identification as Salmonella, STECs or Listeria Monocytogenes of a colony isolated on a plated growth medium.
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