This kit is based on ISO13136 indications and when paired to an appropriate DNA extraction kit (e.g. Generon FASTfood - Cat# EXD009) it allows for the detection of the STEC serotypes indicated in this norm (namely O157, O111, O26, O103, O145) in less than 2 hours. STEC positives can be detected in enriched media broth or in water samples collection filters. The kit in less than 30 minutes, without any DNA extraction step, allows also for the identification as STEC positive of a colony isolated on a plated growth medium. The kit contains seven ready-to-use 2-plex PCR mixes conveniently pre-aliquoted in 8-wells PCR strips: MIX1 [O157 (FAM) IAC (HEX)] - MIX2 [O111 (FAM) IAC (HEX)] - MIX3 [O26 (FAM) IAC (HEX)] - MIX4 [O103 (FAM) IAC (HEX)] - MIX5 [O145 (FAM) IAC (HEX)]. The kit is fully compliant with ISO13136 thus it can be easily accredited in a ISO17025 laboratory. Minimum of order 48 strips.
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