Whatever is the virus to detect, the process of virions collection and concentration is the most critical experimental step to validate. This kit uses the detection of a surrogate virus (FCV) as a tool to perform a process validation according to ISO15216. The kit detects also a synthetic extraction control (Intype IC-RNA from INDICAL) and thus can be used either for the process validation and for the routine process control. The kit includes: a 2-plex oligo mix deteting FCV (FAM) and Intype (HEX) - a prime quality one-step RTqPCR mastermix - a titrated FCV solution to perform the study of spike/recovery.
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