This kit is based on ISO13136 indications and when paired to an appropriate DNA extraction kit (e.g. Generon FASTfood - Cat# EXD009) it allows for the detection of STEC in less than 2 hours. STEC can be detected in enriched media broth or in water samples collection filters. The kit in less than 30 minutes, without any DNA extraction step, allows also for the identification as STEC of a colony isolated on a plated growth medium. The kit contains two 2-plex PCR mixes for 50 tests each: MIX1 [stx1 (FAM) stx2 (HEX)] - MIX2 [eae (FAM) IAC (HEX)].
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